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ZytoFast® Products for CISH Analysis
Achieving Chromogenic in situ Hybridization Results in just 4 Hours!
The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-k and Ig-l light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples.
ZytoFast®: Outstandingly fast CISH Optimized protocols and faster tissue penetration due to short oligonucleotide probes of the ZytoFast® system, make the ZytoFast® CISH procedure outstandingly fast.
Single color results can be achieved within just 4 hours, hands-on time is about 2 hours!
High Sensitivity and Specificity All ZytoFast® probes are tagged using the unique ZytoFast® HighTag System providing improved signal intensity! High specificity without risk of cross-hybridizations is obtained due to optimized oligonucleotide probes.
Advantages of CISH
Simultaneous observation of tissue morphology and CISH signals
No risk of false positives due to mispriming or contamination as with PCR
The ZytoFast® system uses oligonucleotide probes tagged with Biotin or Digoxigenin (1) which are detected using enzyme-conjugated antibodies or streptavidin targeting the tags (2). The enzymatic reaction of chromogenic substrates (3), e.g. BCIP/NBT or AEC, leads to the formation of strong colour precipitates that can be visualized by light microscopy.
Achieving Chromogenic in situ Hybridization Results in just 4 Hours!
The ZytoFast® products are designed for outstandingly fast detection and discrimination of human pathogen viruses, e.g. HPV, EBV, CMV, and the determination of lymphocyte clonality by detecting Ig-k and Ig-l light chain RNA by Chromogenic in situ Hybridization (CISH) in formalin-fixed, paraffin-embedded tissue sections and cell samples.
ZytoFast®: Outstandingly fast CISH Optimized protocols and faster tissue penetration due to short oligonucleotide probes of the ZytoFast® system, make the ZytoFast® CISH procedure outstandingly fast.
Single color results can be achieved within just 4 hours, hands-on time is about 2 hours!
High Sensitivity and Specificity All ZytoFast® probes are tagged using the unique ZytoFast® HighTag System providing improved signal intensity! High specificity without risk of cross-hybridizations is obtained due to optimized oligonucleotide probes.
Advantages of CISH
Simultaneous observation of tissue morphology and CISH signals
No risk of false positives due to mispriming or contamination as with PCR
Easy method comparable to IHC
No costly equipment needed
Ability to test archival specimens
High sensitivity and specificity
The ZytoFast® system uses oligonucleotide probes tagged with Biotin or Digoxigenin (1) which are detected using enzyme-conjugated antibodies or streptavidin targeting the tags (2). The enzymatic reaction of chromogenic substrates (3), e.g. BCIP/NBT or AEC, leads to the formation of strong colour precipitates that can be visualized by light microscopy.
https://www.zytovision.com/ZytoVision
The ZytoFast® system uses oligonucleotide probes tagged with Biotin or Digoxigenin (1) which are detected using enzyme-conjugated antibodies or streptavidin targeting the tags (2). The enzymatic reaction of chromogenic substrates (3), e.g. BCIP/NBT or AEC, leads to the formation of strong colour precipitates that can be visualized by light microscopy.
