Nexcelom Bioscience's Disposable Hemacytometer is an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates.
The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.
How do I Count Cells with a Nexcelom Disposable Hemacytometer?
Step 1. Prepare Sample & Load Hemacytometer
Pipette 20 microliters of sample into the Nexcelom disposable hemacytometer
Step 2. Manually Count Cells in Sample
a. Place the disposable hemacytometer under a microscope with a typical magnification of 100.
b. Focused both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares.
Step 3. Cell Calculations & Disposal of Hemacytometer
a. Multiply the dilution factor by the total number of cells and divide by the sample volume.
b. Dispose the hemacytometer.
Nexcelom Bioscience's Disposable Hemacytometer is an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates.
The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.
How do I Count Cells with a Nexcelom Disposable Hemacytometer?
Step 1. Prepare Sample & Load Hemacytometer
Pipette 20 microliters of sample into the Nexcelom disposable hemacytometer
Step 2. Manually Count Cells in Sample
a. Place the disposable hemacytometer under a microscope with a typical magnification of 100.
b. Focused both onto the grid pattern and the cell particles, and count the total number of cells found in 4 large corner squares.
Step 3. Cell Calculations & Disposal of Hemacytometer
a. Multiply the dilution factor by the total number of cells and divide by the sample volume.
b. Dispose the hemacytometer.
