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ZytoLight® Products for FISH Analysis
Reliable Multi-Target Detection using Fluorescence in situ Hybridization!
ZytoLight® products are designed for the identification of genetic aberrations e.g. translocations, deletions, amplifications, and chromosomal aneuploidies by Fluorescence in situ Hybridization (FISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
High Sensitivity and Specificity ZytoLight® FISH probes are direct labeled using the unique ZytoLight® Direct Label System II providing improved signal intensity. All ZytoLight® single copy (SPEC™) probes are processed by the unique ZytoLight® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
ZytoLight® CEN TM probes hybridize to highly repetitive human satellite DNA sequences of chromosomes producing sharp, bright signals specific for each individual chromosome.
ZytoLight® Kits – Convenient Solutions For making FISH analysis reliable and user-friendly, all ZytoLight® FISH probes can be combined with the ZytoLight® FISH-Tissue Implementation Kit (Z-2028-5/-20) or the ZytoLight® FISH-Cytology Implementation Kit (Z-2099-20), for FISH analyses on cytology specimens. Both Implementation Kits include all necessary pretreatment solutions, wash buffers and DAPI/DuraTect™ -Solution and a detailed protocol to perform successful FISH experiments.
Additionally, for some major targets, complete kits including probes and all necessary reagents are available.
The ZytoLight® system uses direct labeled FISH probes (1), eliminating the need to detect the probes with fluorophore-coupled antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets (2). Due to an exciter filter (3), full-spectrum light, emitted by the microscope lamp (4), is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror (5). The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter (6) reduces the emitted light to a defined wavelength that can be detected.
Reliable Multi-Target Detection using Fluorescence in situ Hybridization!
ZytoLight® products are designed for the identification of genetic aberrations e.g. translocations, deletions, amplifications, and chromosomal aneuploidies by Fluorescence in situ Hybridization (FISH) in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
High Sensitivity and Specificity ZytoLight® FISH probes are direct labeled using the unique ZytoLight® Direct Label System II providing improved signal intensity. All ZytoLight® single copy (SPEC™) probes are processed by the unique ZytoLight® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
ZytoLight® CEN TM probes hybridize to highly repetitive human satellite DNA sequences of chromosomes producing sharp, bright signals specific for each individual chromosome.
ZytoLight® Kits – Convenient Solutions For making FISH analysis reliable and user-friendly, all ZytoLight® FISH probes can be combined with the ZytoLight® FISH-Tissue Implementation Kit (Z-2028-5/-20) or the ZytoLight® FISH-Cytology Implementation Kit (Z-2099-20), for FISH analyses on cytology specimens. Both Implementation Kits include all necessary pretreatment solutions, wash buffers and DAPI/DuraTect™ -Solution and a detailed protocol to perform successful FISH experiments.
Additionally, for some major targets, complete kits including probes and all necessary reagents are available.
For RESEARCH USE ONLY
The ZytoLight® system uses direct labeled FISH probes (1), eliminating the need to detect the probes with fluorophore-coupled antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets (2). Due to an exciter filter (3), full-spectrum light, emitted by the microscope lamp (4), is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror (5). The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter (6) reduces the emitted light to a defined wavelength that can be detected.
https://www.zytovision.com/ZytoVision
The ZytoLight® system uses direct labeled FISH probes (1), eliminating the need to detect the probes with fluorophore-coupled antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets (2). Due to an exciter filter (3), full-spectrum light, emitted by the microscope lamp (4), is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror (5). The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter (6) reduces the emitted light to a defined wavelength that can be detected.
